Journal: Horticulture Research

Article Title: The eTM–miR3699–MAN7 mediated cell wall degradation in regulating embryogenic cell formation during the early stage of somatic embryogenesis in apple

doi: 10.1093/hr/uhaf315

Figure Lengend Snippet: Identification of differentially expressed lncRNAs and mRNAs involved in embryogenic cell formation in M. domestica ‘Gala.’ (a) Initial lncRNA identification was conducted with CNC1, CPC2, and Feelnc. (b) DElncRNAs volcano plot. (c) DEmRNAs volcano plot. (d) KEGG enrichment analysis of DEmRNAs. (e) Heatmap of MdMAN7 (MD15G0020200) under the induction of auxin on Day 0 and Day 8.

Article Snippet: The RT-qPCR procedure was as follows: after starting at 95°C for 30 s, the fusion curve was analyzed after 45 cycles of 95°C for 30 s, 58°C for 15 s, and 72°C for 30 s. The RT-qPCR for lncRNA was performed as the description of lnRcute lncRNA qPCR Kit (Tiangen, Beijing, China).

Techniques:

Journal: Horticulture Research

Article Title: The eTM–miR3699–MAN7 mediated cell wall degradation in regulating embryogenic cell formation during the early stage of somatic embryogenesis in apple

doi: 10.1093/hr/uhaf315

Figure Lengend Snippet: Identification of ceRNAs in the early stage of SE in M. domestica ‘Gala.’ (a) The ceRNA relationships under the treatment of auxin in M. domestica ‘Gala.’ (b) The KEGG pathway enrichment of ceRNAs. (c) The eTM3699–miR3699– MdMAN7 module diagram. (d) Phylogenetic tree analysis of MdMAN7. (e) RT-qPCR analysis of eTM3699, mature miR3699 and MdMAN7 in the early stage of SE in M. domestica ‘Gala.’ Values represent means ± SEs. Statistical significance was assessed by Student’s t -test ( * P < 0.05, ** P < 0.01).

Article Snippet: The RT-qPCR procedure was as follows: after starting at 95°C for 30 s, the fusion curve was analyzed after 45 cycles of 95°C for 30 s, 58°C for 15 s, and 72°C for 30 s. The RT-qPCR for lncRNA was performed as the description of lnRcute lncRNA qPCR Kit (Tiangen, Beijing, China).

Techniques: Quantitative RT-PCR